Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker ab11826 from Abcam

Product Specs
ItemAnti-SC35 antibody [SC-35] - Nuclear Speckle Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab11826
Antibody TypePrimary
CloneSC-35
IsotypeIgG1
HostMouse, Nude mouse
ClonalityMonoclonal
Quantity10 UG, 100 UG
ApplicationsAffinity purification, Immunocytochemistry/ Immunofluorescence, Affinity purification, Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameSRSF2
ReactivityHuman, Mouse, Rat
TargetSC35

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(1) Reduction of oligomer size modulates the competition between cluster formation and phase separation of the tumor suppressor SPOPJournal of Biological ChemistryDecember 1, 2023Nafiseh Sabri, Matthew J. Cuneo, Melissa R. Marzahn, Jihun Lee, Jill J. Bouchard, Ömer Güllülü, Sivaraja Vaithiyalingam, Madeleine B. Borgia, Jeremy Schmit, Tanja Mittag(Cell Signaling Technology, #2276S), Flag (Sigma-Aldrich, #F3165), GAPDH (Abcam, #ab181602), SC35 (Abcam, #ab11826), and PML (Santa Cruz Biotechnology, #sc-966). Horseradish peroxidase-conjugated secondary antibodies

(2) Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagyNature CommunicationsNovember 3, 2023Donghai Cui, Zixiang Wang, Qianli Dang, Jing Wang, Junchao Qin, Jianping Song, Xiangyu Zhai, Yachao Zhou, Ling Zhao, Gang Lu, et al.(1:200 Proteintech 15294-1-AP), rat anti-Lamp2 (1:200 Abcam ab13524), mouse anti-SC35 (1:400 Abcam ab11826). Fluorescent images were captured on a Dragonfly 200 confocal microscopy system (Andor Technology).

(3) Genome-wide analysis of the interplay between chromatin-associated RNA and 3D genome organization in human cellsNature CommunicationsOctober 16, 2023Riccardo Calandrelli, Xingzhao Wen, John Lalith Charles Richard, Zhifei Luo, Tri C. Nguyen, Chien-Ju Chen, Zhijie Qi, Shuanghong Xue, Weizhong Chen, Zhangming Yan, et al.cells were incubated with 1 mL diluted mouse monoclonal anti-SC35 primary antibody (1:250) (Abcam, Cat# ab11826) in 5% BSA at 37 °C for 1 h, and subsequently washed three times with PBST on shaker for 10 min. Cells

(4) Specific Regulation of m(6)A by SRSF7 Promotes the Progression of GlioblastomaGenomics, proteomics & bioinformaticsAugust 1, 2023Cun Y, An S, Zheng H, Lan J, Chen W, Luo W, Yao C, Li X, Huang X, Sun X, et al.HPA038002, Sigma), anti-WTAP (1:500; Cata-log No. ab195380, Abcam), and anti-SC35 (1:200; Catalog No. ab11826, Abcam)] at 4 °C overnight. RNA isolation and qRT-PCR Total RNA was extracted using Trizol reagent

(5) The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcriptsThe Journal of biological chemistryAugust 1, 2023Kases K, Schubert E, Hajikhezri Z, Larsson M, Devi P, Darweesh M, Andersson L, Akusjärvi G, Punga T, Younis S.anti-Flag (mouse, Sigma, M2, F1804), anti-PABPN1 (rabbit, Abcam, ab75855), anti- SC35 (mouse, Abcam, ab11826) diluted in the blocking solution overnight at 4 °C. Proteins were visualized with the FITC- and TRITC-conjugated

(6) Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infectionScientific ReportsJuly 6, 2023Charlotte Luchsinger, KyeongEun Lee, Gonzalo A. Mardones, Vineet N. KewalRamani, Felipe Diaz-Grifferoand cell reagents We used the following mouse monoclonal antibodies: clone SC-35 against SC35 (Cat# ab11826, Abcam), clone 3F8 against CPSF5 (Cat# H00011051-M12, Novus Biologicals), clone A-9 against CPSF7 (Cat#

(7) RNA-mediated demixing transition of low-density condensatesNature CommunicationsApril 27, 2023Taehyun Kim, Jaeyoon Yoo, Sungho Do, Dong Soo Hwang, YongKeun Park, Yongdae Shinsubstitutions. Antibodies Primary antibodies used in this study were mouse monoclonal anti-SRSF2 (Abcam, ab11826, 1:200 dilution), mouse monoclonal anti-NPM1 (Santi Cruz, sc-56622, 1:100 dilution), mouse monoclonal

(8) Nuclear bodies protect phase separated proteins from degradation in stressed proteomebioRxivApril 25, 2023Kwan Ho Jung, Jiarui Sun, Chia-Heng Hsiung, Xiaojun Lance Lance Lian, Yu Liu, Xin Zhang(Invitrogen, PA5–89420, 1:100), rabbit anti-SFPQ (Abcam, ab177149, 1:200), mouse anti-SC35 (Abcam, ab11826, 1:200), rabbit anti-HSP70 (Abcam, ab181606, 1:50). After washing with PBS three times for 10 minutes

(9) HIV-induced membraneless organelles orchestrate post-nuclear entry stepsJournal of molecular cell biologyApril 6, 2023Scoca V, Morin R, Collard M, Tinevez JY, Di Nunzio F.1:500 (Roche #11867423001), anti-CPSF6 1:400 (Novus Biologicals #NBP1-85676), anti-SC35 1:200 (Abcam #ab11826), and anti-LEDGF 1:200 (BD Bioscience #611715). Secondary antibodies used were: donkey anti-rabbit Cy3

(10) A bacterial virulence factor interacts with the splicing factor RBM5 and stimulates formation of nuclear RBM5 granulesScientific reportsDecember 19, 2022Pourpre R, Lakisic G, Desgranges E, Cossart P, Pagliuso A, Bierne H.anti-nucleolin (Santa cruz, sc-13057) and monoclonal antibodies against tubulin (hybridoma E7), SC35 (AbCam, ab11826), FLAG-M2 (Sigma-Aldrich, F1804), Myc (9E10, Santa Cruz Biotechnology, sc-40), HA (6E2, Cell Signaling technology

(11) Small molecule quercetin binds MALAT1 triplex and modulates its cellular functionMolecular Therapy - Nucleic AcidsDecember 13, 2022Isha Rakheja, Asgar Hussain Ansari, Arjun Ray, Dheeraj Chandra Joshi, Souvik Maitiwith PBS and then fixed and permeabilized for immunofluorescence staining with SC35 antibody (Abcam Ab11826) in a humid chamber overnight. The next day, the cells were washed and incubated with a secondary antibody

(12) Cytoplasmic forces functionally reorganize nuclear condensates in oocytesNature CommunicationsAugust 29, 2022Adel Al Jord et al.(1:100; Survival of Motor Neurons, clone 2B1, 05–1532, EMD Millipore), mouse IgG1 anti-SRSF2/SC35 (1:400; ab11826, Abcam), mouse IgG1 anti-TDP-43/TARDBP (1:1500; clone 3H8, MABN45, EMD Millipore), and species-specific

(13) A function for ataxia telangiectasia and Rad3-related (ATR) kinase in cytokinetic abscissioniScienceJuly 15, 2022Luessing J, Okowa CC, Brennan E, Voisin M, Lowndes NF.anti-ATR (C-1),SantaCruz Biotechnology,#sc-515173; RRID: AB_2893291 Mouse-monoclonal anti-SC35,Abcam,#ab11826; RRID: AB_298608 Mouse-monoclonal anti-PML,Abcam,#ab96051; RRID: AB_10679887 Mouse-monoclonal anti-Coilin,Abcam,#

(14) The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein trafficMolecular biology of the cellFebruary 1, 2022Turn RE, Hu Y, Dewees SI, Devi N, East MP, Hardin KR, Khatib T, Linnert J, Wolfrum U, Lim MJ, et al.commercial sources. The following commercial antibodies were used in these studies: SC35 (1:500; Abcam; ab11826), HSP60 (1:1000; Stressgen; ADI-SPA-807), α-tubulin (1:1000; Sigma; T9026), γ-tubulin (1:5000; Sigma

(15) METTL16 exerts an m(6)A-independent function to facilitate translation and tumorigenesisNature cell biologyFebruary 1, 2022Su R, Dong L, Li Y, Gao M, He PC, Liu W, Wei J, Zhao Z, Gao L, Han L, et al.Sigma-Aldrich), METTL3 (1:100, ab195352, Abcam), METTL 14 (1:100, HPA038002, Sigma-Aldrich) and SC35 (1:500, ab11826, Abcam) overnight at 4°C, respectively. After washing with PBS three times, the corresponding fluorescence-labeled

(16) Four-dimensional nuclear speckle phase separation dynamics regulate proteostasisScience advancesJanuary 7, 2022Dion W, Ballance H, Lee J, Pan Y, Irfan S, Edwards C, Sun M, Zhang J, Zhang X, Liu S, et al.blocked with 10% goat serum at room temperature for 1 hour. Primary antibodies against SC35 (Abcam, ab11826), XBP1s (BioLegend, 658802), and SON (Abcam, 121033) were conjugated to Alexa Fluor 488 or Alexa Fluor

(17) Time-dependent effect of 1,6-hexanediol on biomolecular condensates and 3D chromatin organizationGenome BiologyDecember 1, 2021Xinyi Liu, Shaoshuai Jiang, Lin Ma, Jiale Qu, Longying Zhao, Xing Zhu, Junjun Ding(Abcam, ab128874), MED1 (Abcam, ab64965), HP1a (Abcam, ab109028), RING1B (CST, 5694), SC35 (Abcam, ab11826), CTCF (Santa Cruz, sc-271474), SMC1A (Santa Cruz, sc-393171), SMC1A (Bethyl laboratories, A300-055A),

(18) Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerizationThe Journal of biological chemistryNovember 1, 2021Kundinger SR, Dammer EB, Yin L, Hurst C, Shapley S, Ping L, Khoshnevis S, Ghalei H, Duong DM, Seyfried NT.monoclonal anti-SRSF2 (Clone 1Sc-4F11, Millipore Sigma 04-1550), mouse monoclonal anti-pSRSF2 (Abcam ab11826), rabbit polyclonal anti-LUC7L (Thermo Fisher 17085-1-AP), rabbit polyclonal anti-LUC7L3 (Thermo Fisher

(19) Speckles and paraspeckles coordinate to regulate HSV-1 genes transcriptionCommunications biologyOctober 21, 2021Li K, Wang Z.(3800-11700 bp) of human NEAT1v2 overnight at 37 °C and then incubated with anti-SRSF2 antibodies (Abcam, ab11826), anti-EZH2 antibodies (Abcam, ab228697), anti-P300 antibodies (Abcam, ab59240), or anti-CBP antibodies

(20) HIV-1 requires capsid remodelling at the nuclear pore for nuclear entry and integrationPLoS pathogensSeptember 1, 2021Guedán A, Donaldson CD, Caroe ER, Cosnefroy O, Taylor IA, Bishop KN.following primary antibodies: anti-HIV-1 CA (in house), anti-CPSF6 (Atlas; HPA039973) and anti-SC35 (Abcam; ab11826), diluted in 1% DS with 0.5% saponin in PBS (antibody buffer) for 1h at RT. After three washes with

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12/28/2016Good Antibody For Nuclear Speckles Tara VanderweydeWe used this antibody to assess which nuclear substructure was associating with our protein of interest. Antibody worked well at indicated concentrations in rat and mouse cell lines for ... Read Review

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker from Abcam

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Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker from Abcam

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